BL21(DE3)ΔserC is a specialized E. coli host strain engineered for advanced protein engineering applications, for protein phosphorylation, specifically targeting the site-specific incorporation of non-natural amino acids.

Technical Specifications & Mechanism

• Genotype: BL21(DE3) background with a targeted deletion of the serC gene.

• Function: The deletion of the serC gene (encoding phosphoserine aminotransferase) disrupts the endogenous synthesis of phosphoserine from 3-phosphohydroxypyruvate.

• Application: By reducing intracellular phosphoserine levels, this strain minimizes competitive interference, significantly improving the efficiency of incorporating synthetic analogs like phosphoserine or phosphothreonine into recombinant proteins.

Usage Classification

• Research Use Only (RUO): This product is intended strictly for laboratory research and development. It is not designed or approved for use in clinical procedures or any human or animal applications.